Comparison of the Three Phenotypic Methods to Detect Biofilm Production in Coagulase Negative Staphylococci
نویسندگان
چکیده
منابع مشابه
Extracellular Polysaccharide Production and Biofilm Formation by Coagulase-negative Staphylococci
Coagulase-negative Staphylococci (CoNS) form a part of the skin flora and commonly cause nosocomial bloodstream and catheterrelated infections. According to their relatively low virulence, it is important to detect the virulence factors of clinical isolates. The production of extracellular polymers and formation of biofilms belong to virulence factors. We studied clinical isolates of CoNS and c...
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In this study, we determined the accuracy of phenotypic tests (cefoxitin agar dilution, 30 microg-cefoxitin and 1 microg-oxacillin disks) to detect the oxacillin resistance/mecA gene among coagulase-negative staphylococci (CoNS) isolates. The presence of the mecA gene was detected by PCR technique (gold standard). A total of 176 CoNS isolates from blood of hospitalized patients were evaluated. ...
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Coagulase-negative staphylococci (CNS) species identification is still difficult for most clinical laboratories. The scheme proposed by Kloos and Schleifer and modified by Bannerman is the reference method used for the identification of staphylococcal species and subspecies; however, this method is relatively laborious for routine use since it requires the utilization of a large number of bioch...
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Introduction Coagulase negative staphylococci (CoNS) are commensals of non-sterile sites in humans and become pathogenic mostly when the host is immunocompromised by prior diseases or invasive surgical or related procedures [1]. Slime or biofilm production by CoNS has been identified as an important factor in the pathogenesis of infections as bacteria organized in biofilms are protected from th...
متن کاملcomparison of two pcr methods in determining the methicillin-resistant gene in coagulase-negative staphylococci
aims: it is very important to detect the coagulase-negative staphylococci, which produce the hospital infections. being one of the most expensive and time-consuming stages before the polymerase chain reaction (pcr), dna extraction is one of the primary stages of pcr. then, it should be noticed that the elimination of the stage might save time and costs. the aim of this study was to compare two ...
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ژورنال
عنوان ژورنال: Journal of Evolution of Medical and Dental Sciences
سال: 2020
ISSN: 2278-4748,2278-4802
DOI: 10.14260/jemds/2020/299